Polygalacturonase from Tomato Fruits Infected with Penicillium funiculosum Thom.

An Antiviral Substance from Penicillium Funiculosum

1. Helenine prevents the establishment in mice of passive viral immunity by anti-Semliki serum of swine, rabbit, or guinea pig origin. 2. A period of 12 days must elapse, between the antiviral serum administration and challenge with virus, for prevention of the establishment of passive immunity to become apparent. This period is believed to correspond to that in which injected antibody persists...

Characterisation of Partially Purified Cell Wall-degrading Enzymes: Polygalacturonase and Cellulase from Tomato Fruits Degraded by Aspergillus Niger

Aspergillus niger is a soil saprobe and produces a wide array of hydrolytic and oxidative enzymes and cell wall degrading enzymes. An investigation on the various properties of partially purified polygalacturonase and cellulase enzymes extracted from tomato fruits deteriorated by Aspergillus niger was carried out in this study. The results obtained shows that temperature, pH and substrate conce...

Polygalacturonase in normal and abnormal tomato fruit.

Silencing polygalacturonase expression inhibits tomato petiole abscission.

Virus-induced gene silencing (VIGS) was used as a tool for functional analysis of cell wall-associated genes that have been suggested to be involved in leaf abscission. Tobacco rattle virus is an effective vector for VIGS in tomato (Lycopersicon esculentum). Silencing was more efficient when the plants were grown at 22 degrees C than when they were grown at 20 degrees C or 25 degrees C. The pho...

GH10 xylanase D from Penicillium funiculosum: biochemical studies and xylooligosaccharide production

BACKGROUND The filamentous fungus Penicillium funiculosum produces a range of glycoside hydrolases (GH). The XynD gene, encoding the sole P. funiculosum GH10 xylanase described so far, was cloned into the pPICZαA vector and expressed in methylotrophe yeast Pichia pastoris, in order to compare the results obtained with the P. funiculosum GH11 xylanases data. RESULTS High level expression of re...